Karakterisasi dan Uji Aktivitas Antioksidan Kitosan Cangkang Udang Windu (Penaeus Monodon) dengan Deproteinasi Metode Enzimatis
DOI:
https://doi.org/10.32583/far.v14i3.3629Keywords:
antioksidan, deproteinasi, enzim, kitosanAbstract
Kitosan diperoleh dari senyawa kitin yang terdapat dalam cangkang udang windu (Penaeus monodon). Tujuan dari penelitian ini yaitu membandingkan kualitas kitosan antara hasil deproteinasi metode basa dengan metode enzimatik. Ekstraksi kitosan dari cangkang udang melalui peroses demineralisasi, deproteinasi, dan deasetilasi. Pengujian karakterisasi kitosan telah memenuhi syarat sesuai SNI No.7949-2013. Rendemen hasil ekstraksi KDK dan KDE berturut-turut sebesar 19,8% dan 24,8%. Kadar air KDK adalah 4,3% dan KDE 8,8%, kadar abu KDK sebesar 1,5% dan KDE 1,11%, kadar nitrogen KDK sebesar 1,11 % dan KDE sebesar 1,63%. Derajat deasetilasi KDK sebesar 81,61% dan KDE sebesar 78,63%. Spektrum infra merah KDK dan KDE menunjukkan pita serapan yang memiliki karakteristik seperti kitosan komersil. Nilai IC50 KDK sebesar 118,42±1,92 ppm dan KDE 107,12±1,26 ppm.
References
Ahmad, S.I., Ahmad, R., Khan, M.S., Kant, R.; Shahid, S., Gautam, L., Hasan, G.M., Hassan, I. (2020). Chitin and its derivatives: Structural properties and biomedical applications. Int. J. Biol. Macromol. 164, 526–539.
Aldila, H., Asmar, Fabiani, V.A., Dalimunthe, D.Y., Irwanto, R. (2020). The effect of deproteinization temperature and NaOH concentration on deacetylation step in optimizing extraction of chitosan from shrimp shells waste. IOP Conf. Ser. Earth Environ. Sci. 599, 012003
Ardean, C., Davidescu, C. M., Nemeş, N. S., Negrea, A., Ciopec, M., Duteanu, N., Negrea, P., Duda-Seiman, D., & Musta, V. (2021). Factors Influencing the Antibacterial Activity of Chitosan and Chitosan Modified by Functionalization. International journal of molecular sciences, 22(14), 7449. https://doi.org/10.3390/ijms22147449
Bradauskiene, V., Vaiciulyte-Funk, L., Cernauskas, D., Dzingeleviciene, R., Lima, J.P.M., Bradauskaite, A., Tita, M.A. (2022). The Efficacy of Plant Enzymes Bromelain and Papain as a Tool for Reducing Gluten Immunogenicity from Wheat Bran. Processes, 10, 1948
Imtihani, H. N., & Permatasari, S. N. (2020). Sintesis dan Karakterisasi Kitosan dari Limbah Cangkang Udang Kaki Putih (Litopenaeus vannamei). Jurnal SIMBIOSA, 2(9).
Mathew, G.M., Sukumaran, R.K., Sindhu, R., Binod, P., Pandey, A. (2022). Microbes for the Synthesis of Chitin from Shrimp Shell Wastes. In Application of Microbes in Environmental and Microbial Biotechnology. Environmental and Microbial Biotechnology. Springer: Singapore. pp. 445–471
Mohan K, Muralisankar T, Jayakumar R, Gandhi CR. (2021) A study on structural comparisons of α-chitin extracted from marine crustacean shell waste. Carbohydr Polym Technol Appl. 2:100037
Muthu, M., Gopal, J., Chun, S., Devadoss, A. J. P., Hasan, N., & Sivanesan, I. (2021). Crustacean Waste-Derived Chitosan: Antioxidant Properties and Future Perspective. Antioxidants, 10(2), 228. https://doi.org/10.3390/antiox10020228
Pohling, J., Dave, D., Liu, Y., Murphy, W., Trenholm, S. 2021. Two-step demineralization of shrimp (Pandalus Borealis) shells using citric acid: An environmentally friendly, safe and cost-effective alternative to the traditional approach. Green Chem. 24, 1141–1151
Pratiwi, S. N., Utami, N., Damayanti, N. (2022). Karakterisasi Kitosan Dan Pembuatan Nanopartikel Kitosan Dari Cangkang Pupa Black Soldier Fly (Hermetia Illucens) Extraction Chitosan And Characterization Nanoparticle Chitosan From Pupae Sheels Of Black Soldier Fly (Hermetia illucens). Medical Sains : Jurnal Ilmiah Kefarmasian (Vol. 7, Issue 4).
Rashmi, S.H., Biradar, B., Maladkar, K., Kittur, A.A. (2016). Extraction of Chitin from Prawn Shell and Preparation of Chitosan. Res. J. Chem. Environ. Sci. 4, 70–73. A
Rasweefali M., Sabu S, Sunooj KV, Sasidharan A, Xavier KAM. (2021). Consequences of chemical deacetylation on physicochemical, structural and functional characteristics of chitosan extracted from deep-sea mud shrimp. Carbohydr Polym Technol Appl. 2:100032
Shi, W., Ching, Y.C., Chuah, C.H. (2021). Preparation of aerogel beads and microspheres based on chitosan and cellulose for drug delivery: A review. Int. J. Biol. Macromol. 170, 751–767.
Tanasale, M. F. J. D. P., Bijang, C. M., & Rumpakwara, E. (2019). Preparation of Chitosan with Various Molecular Weight and Its Effect on Depolymerization of Chitosan with Hydrogen Peroxide using Conventional Technique. International Journal of ChemTech Research, 12(01), 112–120. https://doi.org/10.20902/ijctr.2019.120113
Thornber, K., Verner-Jeffreys, D., Hinchliffe, S., Rahman, M.M., Bass, D. and Tyler, C.R. (2020), Evaluating antimicrobial resistance in the global shrimp industry. Rev Aquacult, 12: 966-986.
Utami, N., & Damayanti, P. N. (2023). Phytochemical Analysis, Antioxidant Activity and Ftir Spectroscopic Analysis of Red Leaf Lettuce and Green Leaf Lettuce (Lactuca Sativa L.). Indian Drugs, 60(5), 50–56. https://doi.org/10.53879/id.60.05.13378
Utami, N., Istiningrum, R. B., Rusdiarso, B., & Nuryono, N. (2021). Recovery of Au(III) from Gold Mining Rock with Silica/Chitosan Coated on Iron Sand Magnetic Material. Key Engineering Materials, 884, 90–97.
Varlamov, V.P., Il’Ina, A.V., Shagdarova, B.T., Lunkov, A.P., Mysyakina, I.S. (2020). Chitin/Chitosan and Its Derivatives: Fundamental Problems and Practical Approaches. Biochemistry 85, 154–176.
Younes, I., Rinaudo, M. (2015). Chitin and Chitosan Preparation from Marine Sources. Structure, Properties and Applications. Mar. Drugs. 13, 1133–1174
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